Tag Archives: Genome sequencing

Sequencing genomes with the Museum’s Frozen Collection | Digital Collections Programme

1) museum species

To commemorate the Wellcome Trust Sanger Institute turning 25 in 2018, the Institute and its collaborators are sequencing 25 new genomes of species that reside in the UK and represent the richness of species in this country.  Continue reading

Identifying microbial DNA | The Microverse

Volunteer Stephen Chandler tells us how he has been supporting The Microverse project by using computer software to identify the taxonomic groupings of the DNA sequences revealed in the sequencing machine.

Due to the size of microorganisms, we have until recent years relied on microscopes to identify different species. The advancement of scientific technologies however has made it possible for scientists to extract DNA from microorganisms, amplify that DNA into large quantities and then put the samples into a sequencing machine to reveal the genetic sequences. In The Microverse project, my role begins when the sequencer has finished processing the samples.

A raw data file from the MiSeq machine

A raw data file from the MiSeq machine

When the gene sequencer has finished decoding the PCR products it creates a file much like a typical excel file. The main difference is that this file can be incredibly large as it contains millions of DNA sequences belonging to hundreds if not thousands of species. This requires a powerful computer to run the analysis to identify what is in the sample.

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